Neurological Disease

Human DRG action potential firing (Electrophysiology – current clamp)

• Rheobase
• Drug inhibition of action potentials as a function of concentration and firing frequency

Drug selectivity (Calcium Imaging with Electrical Field Stimulation)

• Drug selectivity for specific neuronal subpopulations
  • Cells expressing TTX-S vs. TTX-R
  • A delta-like vs. C-like neurons
  • Cold- vs. heat-sensing neurons

Drug selectivity (Calcium Imaging)

• Drug selectivity for specific neuronal subpopulations
  • TRPV1
  • TRPA1
  • TRPM8
  • ASIC/TRPV1
  • P2X
  • Opioid receptors

IC₅₀ determination (Electrophysiology – voltage clamp)

• Na_v (total)
• Na_v 1.7
• Na_v 1.8
• TRPV1
• TRPA1
• K_v
• Ca_v