In this study, we describe the high content imaging-based profiling of human sensory neurons under normal and inflammatory conditions using a combination of electrical field stimulation (EFS) and florescence-based calcium imaging. This approach allows the human-specific profiling of new drug candidates in functionally defined sub-populations of cells, improving the ability to select human-active drugs for clinical trials.

AUTHORS: Yannick Miron, Jingming Zhang, Guy Page, Claudio Ghetti, Bryan A. Copits, Steve Davidson, Robert W. Gereau IV, Paul Miller and Andre Ghetti