MEASURING NEUROTOXICITY iN COMPOUNDS USING PERIPHERAL SENSORY NEURONS
The neurotoxicity of compounds on peripheral sensory neurons can be evaluated with human dorsal root ganglia (DRG) cultures at AnaBios. Clinical use of chemotherapeutic agents can be limited by the development of painful sensory neuropathy.
Dissociated human DRG neuronal culture can be used as an in-vitro model of chemotherapy-induced peripheral neuropathy. Multiple day treatment of human DRG neuronal cultures by oxaliplatin leads to neuronal death.
Figure 1: Cell Permeabilization of Oxaliplatin Treated Human DRG Cultures
Figure 1 (above) shows a human DRG neuronal culture treated for 72 hours by vehicle (A), vehicle (B) followed by 0.1% saponin, 50µM oxaliplatin (C) and 500µM oxaiplatin (D). Viability and cell permeabilization were assayed by calcein (green) and ethidium homodimer (red) fluorescence.
SPINAL CORD NEUROTOXICITY
AnaBios probes compound effects on spinal cord neuroexcitability through imaging of spontaneous calcium transients in organotypic slices from recovered human spinal cord. The effect of sodium channel inhibitors was evaluated by measuring the inhibition of action potential dependent calcium spikes in the spinal cord dorsal horn.
Figure 2: Effect of carbamazepine & tetrodotoxin on spinal cord spontaneous calcium transients
In Figure 2 (above), organotypic human spinal cord slice loaded with Fluo-8 exhibits spontaneous calcium activity in the dorsal horn. The figure shows spontaneous calcium transients in imaged individual neurons. Calcium traces from separate neurons are stacked along the y-axis. Application of escalating concentrations (3µM, 10µM, 30µM) of carbamazepine reduce the frequency of spontaneous calcium spikes. Tetrodotoxin at 1µM is applied at the end of the assay.
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